白介素1受體拮抗劑(IL1RA)活性蛋白
Active Interleukin 1 Receptor Antagonist (IL1RA)
IL1RN; IL1-RA; ICIL1RA; IL1ra3; IL1F3; IRAP;IL-1RA; Interleukin-1 Family Member 3; IL1 inhibitor; Anakinra
- 編號APA223Hu61
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份磷酸鹽緩沖液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性狀凍干粉
- 純度> 95%
- 等電點5.4
- 應用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. Gene Sequencing (extract)
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Figure. SDS-PAGE
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Sample: Recombinant IL1RA, Human;
Antibody: Rabbit Anti-Human IL1RA Ab (PAA223Hu06)
Figure. Western Blot
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通過ISO 9001、ISO 13485質量體系認證
活性實驗
IL-1RA is an agent that binds non-productively to the cell surface?interleukin-1 receptor?(IL-1R), the same receptor that binds?interleukin 1?(IL-1), preventing IL-1 from sending a signal to that cell. Besides, Interleukin 1 Alpha (IL-1a) has been identified as an interactor of IL-1RA, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL-1RA and recombinant human IL-1a. Briefly, IL1RA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to IL-1a-coated microtiter wells and incubated for 2 h at 37℃. Wells were washed with PBST and incubated for 1 h with anti-IL-1RA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL-1RA and IL-1a was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of IL-1RA with IL-1a.
用法
Reconstitute in 10mM PBS (pH7.6) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| APA223Hu01 | 白介素1受體拮抗劑(IL1RA)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA223Hu01 | 白介素1受體拮抗劑(IL1RA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA223Hu61 | 白介素1受體拮抗劑(IL1RA)活性蛋白 | Cell?culture;?Activity?Assays. |
| EPA223Hu61 | 白介素1受體拮抗劑(IL1RA)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA223Hu01 | 白介素1受體拮抗劑(IL1RA)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA223Hu06 | 白介素1受體拮抗劑(IL1RA)多克隆抗體 | WB; IHC; ICC; IP. |
| FAA223Hu01 | 抗白介素1受體拮抗劑(IL1RA)多克隆抗體 | Flow cytometry. |
| LAA223Hu71 | 白介素1受體拮抗劑(IL1RA)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| LAA223Hu81 | 白介素1受體拮抗劑(IL1RA)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| MAA223Hu26 | 白介素1受體拮抗劑(IL1RA)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA223Hu22 | 白介素1受體拮抗劑(IL1RA)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA223Hu24 | 白介素1受體拮抗劑(IL1RA)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA223Hu25 | 白介素1受體拮抗劑(IL1RA)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA223Hu21 | 白介素1受體拮抗劑(IL1RA)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA223Hu27 | 白介素1受體拮抗劑(IL1RA)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA223Hu23 | 白介素1受體拮抗劑(IL1RA)單克隆抗體 | WB; IHC; ICC; IP. |
| LAA223Hu72 | 白介素1受體拮抗劑(IL1RA)單克隆抗體(生物素標記) | WB; IHC; ICC. |
| HEA223Hu | 白介素1受體拮抗劑(IL1RA)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA223Hu | 白介素1受體拮抗劑(IL1RA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA223Hu | 白介素1受體拮抗劑(IL1RA)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSA223Hu01 | 白介素1受體拮抗劑(IL1RA)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA223Hu01 | 白介素1受體拮抗劑(IL1RA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
| The Veterinary Journal | Mucosal imbalance of interleukin-1β and interleukin-1 receptor antagonist in canine inflammatory bowel disease [ScienceDirect: S1090023312000962] |
| Daily quercetin supplementation over a period of two weeks results in a moderate accumulation of total plasma flavonols in horses [Researchgate:Source] | |
| Journal of Equine Veterinary Science | Synovial Fluid and Serum Concentrations of Interleukin-1 Receptor Antagonist and Interleukin-1 [Science: Article] |
| Journal of Equine Veterinary Science | A Two-Week Quercetin Supplementation in Horses Results in Moderate Accumulation of Plasma Flavonol Concentrations [Science: Article] |
| Scientific publications in peer reviewed journals | Evidence-based review of efficacy and adverse effects of joint medication and evaluation of synovial fluid and serum markers for osteoarthritis in the horse [receive:101658] |
| Multiple Sclerosis and Related Disorders | Interleukin 1 receptor antagonist and 2′-5′-oligoadenylate synthetase-like molecules as novel biomarkers for multiple sclerosis patients in Bahrain [pubmed:29141788] |
| Advances in Medical Sciences | A similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmatics [pubmed:28822267] |
| Archives of Medical Research | Winter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young?… [Pubmed:29379533] |
| Journal of Veterinary?Internal?Medicine | Effect of interleukin‐1β on occludin mRNA expression in the duodenal and colonic mucosa of dogs with inflammatory bowel disease [Pubmed:29572935] |
| Frontiers in Pharmacology | M10, a Myricetin-3-ObD-Lactose Sodium Salt, Prevents Ulcerative Colitis Through Inhibiting Necroptosis in Mice [Pubmed: 33041798] |
