白介素1受體拮抗劑(IL1RA)活性蛋白
Active Interleukin 1 Receptor Antagonist (IL1RA)
IL1RN; IL1-RA; ICIL1RA; IL1ra3; IL1F3; IRAP;IL-1RA; Interleukin-1 Family Member 3; IL1 inhibitor; Anakinra
- 編號(hào)APA223Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 95%
- 等電點(diǎn)8.5
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 1920 ¥ 4800 ¥ 9600 ¥ 28800 ¥ 72000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Gene sequencing
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SDS-PAGE圖
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Figure. Western Blot; Sample: Recombinant IL1RA, Mouse.
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
IL1RA (interleukin-1 receptor antagonist) is an agent that binds to the cell surface?interleukin-1 receptor?(IL-1R), which would prevent IL-1 from intracellular signal transduction. Besides, mouse IL1R1 and rat IL1R1 share similarities in amino acid sequence with the identity of 85.6%. Thus, a binding ELISA assay was constructed to detect the association of recombinant mouse IL1RA with recombinant rat IL1R1. Briefly, IL1RA were diluted serially in PBS with 0.1%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1R1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL1RA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL1RA with IL1R1 was shown in Figure 1 and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APA223Mu01 | 白介素1受體拮抗劑(IL1RA)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA223Mu02 | 白介素1受體拮抗劑(IL1RA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA223Mu01 | 白介素1受體拮抗劑(IL1RA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA223Mu02 | 白介素1受體拮抗劑(IL1RA)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA223Mu01 | 白介素1受體拮抗劑(IL1RA)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA223Mu71 | 白介素1受體拮抗劑(IL1RA)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAA223Mu81 | 白介素1受體拮抗劑(IL1RA)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| SEA223Mu | 白介素1受體拮抗劑(IL1RA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA223Mu | 白介素1受體拮抗劑(IL1RA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA223Mu01 | 白介素1受體拮抗劑(IL1RA)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | 自己動(dòng)手制作ELISA試劑盒的主要材料 |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| The Veterinary Journal | Mucosal imbalance of interleukin-1β and interleukin-1 receptor antagonist in canine inflammatory bowel disease [ScienceDirect: S1090023312000962] |
| Daily quercetin supplementation over a period of two weeks results in a moderate accumulation of total plasma flavonols in horses [Researchgate:Source] | |
| Journal of Equine Veterinary Science | Synovial Fluid and Serum Concentrations of Interleukin-1 Receptor Antagonist and Interleukin-1 [Science: Article] |
| Journal of Equine Veterinary Science | A Two-Week Quercetin Supplementation in Horses Results in Moderate Accumulation of Plasma Flavonol Concentrations [Science: Article] |
| Scientific publications in peer reviewed journals | Evidence-based review of efficacy and adverse effects of joint medication and evaluation of synovial fluid and serum markers for osteoarthritis in the horse [receive:101658] |
| Multiple Sclerosis and Related Disorders | Interleukin 1 receptor antagonist and 2′-5′-oligoadenylate synthetase-like molecules as novel biomarkers for multiple sclerosis patients in Bahrain [pubmed:29141788] |
| Advances in Medical Sciences | A similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmatics [pubmed:28822267] |
| Archives of Medical Research | Winter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young?… [Pubmed:29379533] |
| Journal of Veterinary?Internal?Medicine | Effect of interleukin‐1β on occludin mRNA expression in the duodenal and colonic mucosa of dogs with inflammatory bowel disease [Pubmed:29572935] |
| Frontiers in Pharmacology | M10, a Myricetin-3-ObD-Lactose Sodium Salt, Prevents Ulcerative Colitis Through Inhibiting Necroptosis in Mice [Pubmed: 33041798] |
