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白介素1受體Ⅰ(IL1R1)活性蛋白

Active Interleukin 1 Receptor Type I (IL1R1)

CD121a; CD121-A; IL1-R1; IL1R; IL1RA; P80; CD121 Antigen-Like Family Member A; Interleukin-1 Receptor Alpha

  • 白介素1受體Ⅰ(IL1R1)活性蛋白 產(chǎn)品包裝(模擬)
  • 白介素1受體Ⅰ(IL1R1)活性蛋白 產(chǎn)品包裝(模擬)
  • APA066Ra02.jpg SDS-PAGE圖
  • 白介素1受體Ⅰ(IL1R1)活性蛋白 Figure. Western Blot; Sample: Recombinant IL1R1, Rat.
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

活性實驗

IL1R1 (Interleukin 1 Receptor Type I), also known as CD121a, is an important mediator involved in many cytokine induced immune and inflammatory responses. It belongs to the?interleukin-1 receptor?family, and is a receptor for interleukin 1 alpha (IL1A), interleukin 1 beta (IL1B), and interleukin 1 receptor antagonist (IL1RA). Besides, mouse IL1B shares 87.0% AA sequence identity with rat IL1B, suggesting the exist of cross-species activity. Thus, a binding ELISA assay was constructed to detect the association of recombinant rat IL1R1 with recombinant mouse IL1B. Briefly, IL1R1 were diluted serially in PBS with 0.1%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1B-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL1R1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL1R1 with IL1B was shown in Figure 1 and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。

相關(guān)產(chǎn)品

編號 適用物種：Rattus norvegicus (Rat，大鼠) 應用(僅供研究使用，不用于臨床診斷！)
APA066Ra01 白介素1受體Ⅰ(IL1R1)活性蛋白 Cell?culture;?Activity?Assays.
APA066Ra02 白介素1受體Ⅰ(IL1R1)活性蛋白 Cell?culture;?Activity?Assays.
APA066Ra03 白介素1受體Ⅰ(IL1R1)活性蛋白 Cell?culture;?Activity?Assays.
RPA066Ra03 白介素1受體Ⅰ(IL1R1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA066Ra01 白介素1受體Ⅰ(IL1R1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA066Ra02 白介素1受體Ⅰ(IL1R1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA066Ra03 白介素1受體Ⅰ(IL1R1)多克隆抗體 WB; IHC; ICC; IP.
PAA066Ra02 白介素1受體Ⅰ(IL1R1)多克隆抗體 WB; IHC; ICC; IP.
PAA066Ra01 白介素1受體Ⅰ(IL1R1)多克隆抗體 WB; IHC; ICC; IP.
FAA066Ra03 抗白介素1受體Ⅰ(IL1R1)多克隆抗體 Flow cytometry.
LAA066Ra71 白介素1受體Ⅰ(IL1R1)多克隆抗體(生物素標記) WB; IHC; ICC.
SEA066Ra 白介素1受體Ⅰ(IL1R1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.

參考文獻

雜志 參考文獻
Clinical & Experimental Immunology In vivo extravasation induces expression of IL-1R1 in human neutrophils [Wiley: source]
PLos One The Gene Expression Analysis of Blood Reveals S100A11 and AQP9 as Potential Biomarkers of Infective Endocarditis [Plosone: 0031490]
Journal of Clinical Apheresis Kinetics of the soluble IL-1 receptor type I during treatment with an LCAP filter in patients with inflammatory bowel disease [PubMed: 22267087]
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source]
中國當代兒科雜志 IL1R1 基因多態(tài)性與兒童哮喘的相關(guān)性 [article:13877]
STEM CELLS AND DEVELOPMENT IL-1α regulates osteogenesis and osteoclastic activity of dental follicle cells via JNK and p38 MAPK pathways [Pubmed: 33107399]
Journal of Integrative Medicine Salvadora persica extract attenuates cyclophosphamide-induced hepatorenal damage by modulating oxidative stress, inflammation and apoptosis in rats [Pubmed:35643766]
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