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白介素15(IL15)活性蛋白

Active Interleukin 15 (IL15)

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活性實驗

Interleukin 15 (IL15) is a widely expressed cytokine that is structurally and functionally related to IL2, which plays an important role in many immunological diseases. IL15 also regulates?T?and?natural killer?(NK) cell activation and proliferation. The activity of recombinant human IL15 was measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. MO7e cells were seeded into triplicate wells of 96-well plates at a density of 30,000 cells/well in RPMI-1640 with the addition of various concentrations of rhIL15. After incubated for 72h,?cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 °C. Cell proliferation of MO7e cells after incubation with rhIL15 for 72h observed by inverted microscope was shown in Figure 1. The dose-effect curve of rhIL15 was shown in Figure 2. It was obvious that rhIL15 significantly promoted cell proliferation of MO7e cells .The EC50 for this effect is typically 0.094 ug/ml.

Figure 2. The dose-effect curve of rhIL15 on MO7e cells

用法

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

儲存

避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產生。保質期內,在適當?shù)臈l件下存儲,損失率低于5%。

相關產品

編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
APA061Hu01 白介素15(IL15)活性蛋白 Cell?culture;?Activity?Assays.
RPA061Hu01 白介素15(IL15)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA061Hu01 白介素15(IL15)多克隆抗體 WB; IHC; ICC; IP.
LAA061Hu71 白介素15(IL15)多克隆抗體(生物素標記) WB; IHC; ICC.
RAA061Hu21 白介素15(IL15)重組抗體 WB; IF; ICC; IHC; IP; FCM.
MAA061Hu21 白介素15(IL15)單克隆抗體 WB
MAA061Hu22 白介素15(IL15)單克隆抗體 WB; IHC; ICC; IP.
SEA061Hu 白介素15(IL15)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA061Hu 白介素15(IL15)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
IGA061Hu21 白介素15(IL15)人基因嵌入小鼠 動物實驗,陽性對照。

參考文獻

雜志 參考文獻
Endocrine Expression of interleukin-15 and inflammatory cytokines in skeletal muscles of STZ-induced diabetic rats: effect of resistance exercise training [Pubmed: 24006180]
Endocrine Time course of IL-15 expression after acute resistance exercise in trained rats: effect of diabetes and skeletal muscle phenotype [Pubmed:25522723]
Med Sci Moni miR-15b is Downregulated in Myasthenia Gravis Patients and Directly Regulates the Expression of Interleukin-15 (IL-15) in Experimental Myasthenia Gravis Mice [PubMed: 26087886]
Exercise physiology Effects of intrinsic aerobic capacity, aging and physical activity on interleukin-15 protein level in serum and skeletal muscle [Dspace: Handle]
Biotechnology Letters Novel adjuvant for immunization against tuberculosis: DNA vaccine expressing Mycobacterium tuberculosis antigen 85A and interleukin-15 fusion product elicits strong immune responses in mice [s10529-017-2342-1]
Journal?of?Controlled?Release Interleukin-15 and cisplatin co-encapsulated thermosensitive polypeptide hydrogels for combined immuno-chemotherapy. [pubmed:28408199]
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