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白介素17C(IL17C)活性蛋白

Active Interleukin 17C (IL17C)

Cytokine CX2

  • 白介素17C(IL17C)活性蛋白 產(chǎn)品包裝(模擬)
  • 白介素17C(IL17C)活性蛋白 產(chǎn)品包裝(模擬)
  • APD347Hu01.jpg Figure. SDS-PAGE
  • 白介素17C(IL17C)活性蛋白 Figure. Western Blot; Sample: Recombinant IL17C, Human.
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

活性實驗

IL17C (Interleukin-17C) is a T cell-derived cytokine that shares the sequence similarity with IL17. IL17C is thought to play a crucial role in innate immunity of the epithelium. IL17C binds with affinity to IL17RA. Thus, a binding ELISA assay was constructed to detect the association of rhIL17C with IL17RA. Briefly, rhIL17C were diluted serially in PBS with 0.1% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL17 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL17C with IL17RA was shown in Figure 1 and this effect was in a dose dependent manner.
Figure. The binding activity of IL17C with IL17RA.

用法

PBS或其它。

儲存

避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當?shù)臈l件下存儲,損失率低于5%。

相關產(chǎn)品

編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
RPD347Hu01 白介素17C(IL17C)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APD347Hu01 白介素17C(IL17C)活性蛋白 Cell?culture;?Activity?Assays.
PAD347Hu01 白介素17C(IL17C)多克隆抗體 WB; IHC; ICC; IP.
MAD347Hu21 白介素17C(IL17C)單克隆抗體 WB; IHC; ICC; IP.
SED347Hu 白介素17C(IL17C)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCD347Hu 白介素17C(IL17C)檢測試劑盒(化學發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMD347Hu 白介素17C(IL17C)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
Cell?Mol?Gastroenterol?Hepatol Modulation of Gut Microbiota Composition by Serotonin Signaling Influences Intestinal Immune Response and Susceptibility to Colitis [Pubmed: 30716420]
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