白介素24(IL24)活性蛋白
Active Interleukin 24 (IL24)
C49A; FISP; IL10B; MDA7; Mob-5; ST16; Mda-7; Melanoma Differentiation Association Protein 7; Suppression Of Tumorigenicity 16
- 編號APC064Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 90%
- 等電點8.6
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE; Sample: Active recombinant IL24, Human.
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Figure. Western Blot ; Sample: Recombinant IL24, Human.
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實驗
IL24 (interleukin 24) is a cytokine that belongs to IL10 family. This protein can induce apoptosis selectively in various cancer cells, including ECV304. Thus, inhibition of cell proliferation assay of IL24 was conducted using ECV-304 cells. Briefly,?ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard 1640?prior to the addition of various concentrations of IL24. After incubated for 48h,?cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Inhibition of ECV-304 cells proliferation after incubation with IIL24 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with various concentrations of IL24 for 48h. The mean OD value of ECV-304 assessed by CCK-8 was shown in Figure 2. It was obvious that IL24 significantly decreased cell viability of ECV-304 cells.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPC064Hu01 | 白介素24(IL24)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC064Hu01 | 白介素24(IL24)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAC064Hu01 | 白介素24(IL24)多克隆抗體 | WB; IHC |
| LAC064Hu71 | 白介素24(IL24)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAC064Hu22 | 白介素24(IL24)單克隆抗體 | WB; IHC; ICC; IP. |
| SEC064Hu | 白介素24(IL24)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCC064Hu | 白介素24(IL24)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMC064Hu | 白介素24(IL24)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Experimental & Clinical Cancer Research | Oncolytic adenovirus armed with IL-24 Inhibits the growth of breast cancer in vitro and in vivo [PubMed: PMC3511263] |
| Bioresources and Bioprocessing | Improving the mda-7/IL-24 refolding and purification process using optimized culture conditions based on the structure characteristics of inclusion bodies [Springer:Source] |
| Clin Exp Pharmacol Physiol. | Inhibition of ALDH2 protects PC12 cells against formaldehyde-induced cytotoxicity: involving the protection of hydrogen sulphide. [pubmed:28251688] |
| Theranostics | IL20RA signaling enhances stemness and promotes the formation of an immunosuppressive microenvironment in breast cancer [33456560] |
| BIOLOGICAL CHEMISTRY | IL-24 inhibits the malignancy of human glioblastoma cells via destabilization of Zeb1 [33894112] |
| J Transl Med | Interleukin-24 regulates mucosal remodeling in inflammatory bowel diseases [34078403] |
| Nutrients | Effect of Serum Spermidine on the Prognosis in Patients with Acute Myocardial Infarction: A Cohort Study [Pubmed:35406007] |
