Janus激酶1(JAK1)活性蛋白
Active Janus Kinase 1 (JAK1)
JAK1A; JAK1B; JTK3; Tyrosine-protein kinase JAK1
- 編號(hào)APC551Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 95%
- 等電點(diǎn)6.2
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 1517 ¥ 3792 ¥ 7584 ¥ 22752 ¥ 56880
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Janus Kinase 1, also known as JAK1, is a member of the Janus kinase family, which plays a crucial role in various cellular processes, including signal transduction, immune response, and hematopoiesis. It is reported that the binding of JAK1 to IFNa2 plays an important role in immune response and antiviral defense. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human JAK1 and recombinant mouse IFNa2. Briefly, JAK1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IFNa2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-JAK1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human JAK1 and recombinant mouse IFNa2 was shown in Figure 1, the EC50?for this effect is 0.1 ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPC551Hu01 | Janus激酶1(JAK1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC551Hu01 | Janus激酶1(JAK1)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAC551Hu01 | Janus激酶1(JAK1)多克隆抗體 | WB; IHC; ICC; IP. |
| LAC551Hu71 | Janus激酶1(JAK1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAC551Hu22 | Janus激酶1(JAK1)單克隆抗體 | WB; IHC; ICC; IP. |
| SEC551Hu | Janus激酶1(JAK1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC551Hu | Janus激酶1(JAK1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
