卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)活性蛋白
Active Lecithin Cholesterol Acyltransferase (LCAT)
phosphatidylcholine-sterol O-acyltransferase; Phospholipid-cholesterol acyltransferase
- 編號(hào)APJ516Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)6.3
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure . Gene Sequencing (extract)
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Figure. SDS-PAGE; Sample: Active recombinant LCAT, Mouse.
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Figure. Western Blot; Sample: Recombinant LCAT, Mouse.
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
LCAT (Phosphatidylcholine-sterol acyltransferase) is an enzyme in the extracellular metabolism of plasma lipoproteins, which converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines. It is reported that APOA1 (Apolipoprotein A-I) acts as a cofactor for the LCAT. Thus, a binding ELISA assay was conducted to detect the association of LCAT with APOA1. Briefly, recombinant rat LCAT were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to APOA1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-LCAT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of LCAT with APOA1 was shown in Figure 1 and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPJ516Mu02 | 卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPJ516Mu01 | 卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APJ516Mu01 | 卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAJ516Mu01 | 卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)多克隆抗體 | WB; IHC; ICC; IP. |
| PAJ516Mu02 | 卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)多克隆抗體 | WB; IHC; ICC; IP. |
| LAJ516Mu71 | 卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEJ516Mu | 卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMJ516Mu | 卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Int J Rheum Dis | Plasma phospholipase, γ‐CEHC and antioxidant capacity in fibromyalgia [PubMed: 26585319] |
| Inflammation. | Alteration?of?Lysophosphatidylcholine-Related?Metabolic?Parameters?in the?Plasma?of?Mice?withExperimental?Sepsis. [pubmed:28028754] |
| British journal of cancer | A combined biomarker panel shows improved sensitivity for the early detection of ovarian cancer allowing the identification of the most aggressive type II tumours. [pubmed:28664912] |
| Journal?of?Agricultural?and?Food?Chemistry | Lipid-lowering effects of medium-chain triglyceride-enriched coconut oil in combination with licorice extracts in experimental hyperlipidemic mice [Pubmed: 30244576] |
| Experimental and Therapeutic Medicine | Identification and validation of differentially expressed proteins in serum of CSU patients with different duration of wheals using an iTRAQ labeling, 2D?LC?MS/MS [Doi: 10.3892/etm.2018.6818] |
| British Journal of Cancer | Diagnosis of epithelial ovarian cancer using a combined protein biomarker panel. [Pubmed: 31388184] |
| BIOMEDICINE & PHARMACOTHERAPY | The synergistic mechanism of total saponins and flavonoids in Notoginseng? Safflower pair against myocardial ischemia uncovered by an integrated?… [Pubmed: 32739736] |
| Biomedicine & Pharmacotherapy | Pharmacokinetics/pharmacometabolomics-pharmacodynamics reveals the synergistic mechanism of a multicomponent herbal formula, Baoyuan decoction??- [] |
| Cell Metab | Defects in a liver-bone axis contribute to hepatic osteodystrophy disease progression [Pubmed:35235775] |
