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基質(zhì)金屬蛋白酶7(MMP7)活性蛋白

Active Matrix Metalloproteinase 7 (MMP7)

MPSL1; MAT; PUMP-1; Matrilysin Uterine; Matrilysin; Matrin; Pump-1 Protease; Uterine Metalloproteinase

  • 基質(zhì)金屬蛋白酶7(MMP7)活性蛋白 產(chǎn)品包裝(模擬)
  • 基質(zhì)金屬蛋白酶7(MMP7)活性蛋白 產(chǎn)品包裝(模擬)
  • APA102Hu61.jpg SDS-PAGE圖
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實驗

Matrix Metalloproteinase 7 (MMP7) is a member of the matrix metalloproteinases (MMPs) family which are zinc and calcium dependent endopeptidases. Structurally, MMP-7 is the smallest of the MMPs and consists of two domains: a pro-domain that is cleaved upon activation and a catalytic domain containing the zinc-binding site. MMP-7 (matrilysin) is expressed in epithelial cells of normal and diseased tissues, and is capable of digesting a large series of proteins of the extracellular matrix including collagen IV and X, gelatin, casein, laminin, aggrecan, entactin, elastin and versican. Thus we have chosen casein-zymography to measure the activity of MMP7. Briefly, various concentrations of MMP7 (10ug, 5ug, 1ug, 0.1ug ,0.01ug) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphat-polyacrylamide gel (SDS-PAGE; 15% gels) containing casein (1 mg/ml) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP7 would hydrolyze casein nearby, which was indicated by the white bands on the gel. In this experiment, we use heat-denatured MMP7 protein as negative control, and trypsin (1ug/ml) as positive control. The result was shown in figure 1.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。

相關(guān)產(chǎn)品

編號 適用物種：Homo sapiens (Human，人) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA102Hu01 基質(zhì)金屬蛋白酶7(MMP7)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA102Hu02 基質(zhì)金屬蛋白酶7(MMP7)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA102Hu61 基質(zhì)金屬蛋白酶7(MMP7)活性蛋白 Cell?culture;?Activity?Assays.
EPA102Hu61 基質(zhì)金屬蛋白酶7(MMP7)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA102Hu01 基質(zhì)金屬蛋白酶7(MMP7)多克隆抗體 WB; IHC; ICC; IP.
PAA102Hu02 基質(zhì)金屬蛋白酶7(MMP7)多克隆抗體 WB; IHC; ICC; IP.
MAA102Hu24 基質(zhì)金屬蛋白酶7(MMP7)單克隆抗體 WB; IHC; ICC; IP.
MAA102Hu28 基質(zhì)金屬蛋白酶7(MMP7)單克隆抗體 WB; IHC; ICC; IP.
MAA102Hu22 基質(zhì)金屬蛋白酶7(MMP7)單克隆抗體 WB
MAA102Hu21 基質(zhì)金屬蛋白酶7(MMP7)單克隆抗體 WB
MAA102Hu23 基質(zhì)金屬蛋白酶7(MMP7)單克隆抗體 WB; IHC
MAA102Hu25 基質(zhì)金屬蛋白酶7(MMP7)單克隆抗體 WB; IHC; ICC; IP.
MAA102Hu27 基質(zhì)金屬蛋白酶7(MMP7)單克隆抗體 WB; IHC; ICC; IP.
MAA102Hu29 基質(zhì)金屬蛋白酶7(MMP7)單克隆抗體 WB; IHC; ICC; IP.
LAA102Hu74 基質(zhì)金屬蛋白酶7(MMP7)單克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
SEA102Hu 基質(zhì)金屬蛋白酶7(MMP7)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA102Hu 基質(zhì)金屬蛋白酶7(MMP7)檢測試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA102Hu 基質(zhì)金屬蛋白酶7(MMP7)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
British Journal of Pharmacology A novel, orally active LPA1 receptor antagonist inhibits lung fibrosis in the mouse bleomycin model [PubMed: 20649573]
Neuroimmunomodulation Upregulated Expression of Matrix Metalloproteinases and Tissue Inhibitors of Matrix Metalloproteinases in BALB/c Mouse Brain Challenged with Japanese Encephalitis Virus [Pubmed: source]
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source]
Thromb Haemost. Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke [Pubmed:24671655]
Histol Histopathol Estrogen-deficient osteoporosis enhances the recruitment and activity of osteoclasts by breast cancer cells [PubMed: 26254457]
VirusDisease Circulating levels of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases during Japanese encephalitis virus infection [Pubmed:26925446]
Am J Physiol Lung Cell Mol Physiol Systematic phenotyping and correlation of biomarkers with lung function and histology in lung fibrosis [Pubmed:26993522]
Cellular Physiology and Biochemistry The MiR-495/Annexin A3/P53 Axis Inhibits the Invasion and EMT of Colorectal Cancer Cells [pubmed:29224019]
s-space Thyroid disrupting effects and associated mechanisms of TCPP in GH3 cell line and zebrafish (Danio rerio) larva and adult [10371/137708]
Science Translational Medicine Large-scale proteomics identifies MMP-7 as a sentinel of epithelial injury and of biliary atresia. [pubmed:29167395]
Hepatology Diagnostic Accuracy of Serum Matrix Metalloproteinase‐7 for Biliary Atresia [Pubmed: 30153340]
Journal of Pediatrics Quantification of Serum Matrix Metallopeptide 7 Levels May Assist in the Diagnosis and Predict the Outcome for Patients with Biliary Atresia [Pubmed: 30853207]
Pediatrics Serum MMP-7 in the Diagnosis of Biliary Atresia [Pubmed: 31604829]
Biomed Pharmacother Human umbilical cord blood mononuclear cells protect against renal tubulointerstitial fibrosis in cisplatin-treated rats [Pubmed: 31710895]
LABORATORY INVESTIGATION The synthetic toxin biliatresone causes biliary atresia in mice [Pubmed: 32681026]
diagnostics Interstitial Score and Concentrations of IL-4R|á, PAR-2, and MMP-7 in Bronchoalveolar Lavage Fluid Could Be Useful Markers for Distinguishing Idiopathic Interstitial??- [33924683]
Cancer Cell Int A novel strategy to identify candidate diagnostic and prognostic biomarkers for gastric cancer [34215253]
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