神經(jīng)氨酸酶(NEU)活性蛋白
Active Neuraminidase (NEU)
NEU1; SIAL1; Sialidase 1; Lysosomal Sialidase; Acetylneuraminyl hydrolase; G9 sialidase; N-acetyl-alpha-neuraminidase 1
- 編號APB611Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 90%
- 等電點5.5
- 應用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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SDS-PAGE圖
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Figure. Western Blot; Sample: Recombinant NEU, Human.
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
NEU (Sialidase-1) is an enzyme that catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (CTSA). Thus a binding ELISA assay was conducted to detect the interaction of recombinant human NEU and recombinant human CTSA. Briefly, NEU were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CTSA-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-NEU pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of NEU and CTSA was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPB611Hu05 | 神經(jīng)氨酸酶(NEU)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB611Hu02 | 神經(jīng)氨酸酶(NEU)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB611Hu01 | 神經(jīng)氨酸酶(NEU)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB611Hu03 | 神經(jīng)氨酸酶(NEU)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB611Hu01 | 神經(jīng)氨酸酶(NEU)活性蛋白 | Cell?culture;?Activity?Assays. |
| APB611Hu02 | 神經(jīng)氨酸酶(NEU)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB611Hu04 | 神經(jīng)氨酸酶(NEU)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB611Hu01 | 神經(jīng)氨酸酶(NEU)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB611Hu02 | 神經(jīng)氨酸酶(NEU)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB611Hu71 | 神經(jīng)氨酸酶(NEU)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| LAB611Hu81 | 神經(jīng)氨酸酶(NEU)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| MAB611Hu21 | 神經(jīng)氨酸酶(NEU)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB611Hu | 神經(jīng)氨酸酶(NEU)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| METHODS OF PREVENTING OR TREATING ATHEROSCLEROSIS WITH INHIBITORS OF SPECIFIC ISOENZYMES OF HUMAN NEURAMINIDASE [] |
