核因子κB2(NFkB2)活性蛋白
Active Nuclear Factor Kappa B2 (NFkB2)
LYT-10; H2TF1; Lymphocyte translocation chromosome 10; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 2(p49/P100); Nuclear factor NF-kappa-B p52
- 編號(hào)APB825Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱(chēng)，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)10.0
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 暫無(wú)報(bào)價(jià) 暫無(wú)報(bào)價(jià) 暫無(wú)報(bào)價(jià) 暫無(wú)報(bào)價(jià) 暫無(wú)報(bào)價(jià)
- 欲了解實(shí)際交易價(jià)格和更多情況，請(qǐng)與當(dāng)?shù)亟?jīng)銷(xiāo)商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
Figure. SDS-PAGE
-
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Nuclear Factor kappa B2 (NFkB2), also known as p100, is a subunit of the NF-kappa B (NFkB) family of transcription factors. NFkB2 plays an important role in immune response and inflammation. It is involved in regulating the expression of a variety of genes, including those encoding cytokines, chemokines, adhesion molecules, and acute phase response proteins. RELB-NFkB2 heterodimer regulates the expression of genes associated with immune response and inflammation in cells. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse NFkB2 and recombinant human RELB. Briefly, NFkB2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to RELB-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-NFkB2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse NFkB2 and recombinant human RELB was shown in Figure 1, the EC50 for this effect is 0.23 ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB825Mu01 | 核因子κB2(NFkB2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB825Mu01 | 核因子κB2(NFkB2)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB825Mu02 | 核因子κB2(NFkB2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB825Mu02 | 核因子κB2(NFkB2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB825Mu01 | 核因子κB2(NFkB2)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB825Mu81 | 核因子κB2(NFkB2)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAB825Mu71 | 核因子κB2(NFkB2)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEB825Mu | 核因子κB2(NFkB2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB825Mu | 核因子κB2(NFkB2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
