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脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)活性蛋白

Active Phospholipase A2 Group VII (LpPLA2)

PLA2G7; PAF-AH; PAFAH; Lp-PLA2; LDL-PLA2; Platelet Activating Factor Acetylhydrolase,Plasma; Phospholipase A2,Group VII; LDL-associated phospholipase A2; Phospholipase A2, Lipoprotein Associated

  • 脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)活性蛋白 產(chǎn)品包裝(模擬)
  • 脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)活性蛋白 產(chǎn)品包裝(模擬)
  • 脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)活性蛋白 Figure. Gene Sequencing (Extract)
  • APA867Hu01.png Figure. SDS-PAGE
  • 脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)活性蛋白 WB圖
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

Phospholipase A2 Group VII (PLA2G7) which is also known as Lp-PLA2, is a plasma enzyme bound to lipoproteins: 80% bound to LDL, 15%-20% to HDL, and the remainder to VLDL. It is produced in major by mature macrophages and activated platelets and?catalyzes the degradation of platelet-activating factor to biologically inactive products. Besides, Paraoxonase 1 (PON1) has been identified as an interactor of PLA2G7, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human PLA2G7 and recombinant rat PON1. Briefly, PLA2G7 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to PON1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PLA2G7 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human PLA2G7 and recombinant rat PON1 was shown in Figure 1, the EC50?for this effect is 0.7 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

相關(guān)產(chǎn)品

編號(hào) 適用物種：Homo sapiens (Human，人) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA867Hu02 脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA867Hu03 脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA867Hu04 脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA867Hu01 脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)活性蛋白 Cell?culture;?Activity?Assays.
RPA867Hu01 脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA867Hu01 脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)多克隆抗體 WB; IHC; ICC; IP.
LAA867Hu81 脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)多克隆抗體(異硫氰酸熒光素標(biāo)記) WB; IHC; ICC; IF.
LAA867Hu71 脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
MAA867Hu21 脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)單克隆抗體 WB; IHC; ICC; IP.
MAA867Hu22 脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)單克隆抗體 WB; IHC; ICC; IP.
SEA867Hu 脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA867Hu 脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA867Hu 脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志 參考文獻(xiàn)
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China Pharmacy Influence of Tongfengtai Granules on Inflammatory Factor in Acute Gouty Arthritis Model Rats [Source]
Atherosclerosis Everolimus therapy is associated with reduced lipoprotein-associated phospholipase A2 (Lp-Pla2) activity and oxidative stress in heart transplant recipients. [Pubmed: 23958269]
Journal of Diabetes Research Lipoprotein-Associated Phospholipase A2 Mass Level Is Increased in Elderly Subjects with Type 2 Diabetes Mellitus [Hindawi: 278063]
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Biology Bulletin In vitro lipid-lowering and fibrinolytic effects of regulatory leucine-containing glyprolines in human blood [Springer:Source]
Gynecol Endocrinol. Lipoprotein-associated phospholipase A2 and AGEs are associated with cardiovascular risk factors in women with history of gestational diabetes mellitus. [Pubmed:24397392]
Journal of Diabetes Research Lipoprotein-Associated Phospholipase A 2 Mass Level Is Increased in Elderly Subjects with Type 2 Diabetes Mellitus [Pubmed:24818163]
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Endocrinol Metab (Seoul). Effect of Pitavastatin Treatment on ApoB-48 and Lp-PLA2?in Patients with Metabolic Syndrome: Substudy of PROspective Comparative Clinical Study Evaluating the Efficacy and Safety of PITavastatin in Patients with Metabolic Syndrome [pmc:PMC4803547]
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Biomarker for diagnosis of moyamoya disease [:]
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chinese neurosurgical journal Can admission lipoprotein-associated phospholipase A2 predict the symptomatic cerebral vasospasm following aneurysmal subarachnoid hemorrhage? []
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