S100鈣結(jié)合蛋白A6(S100A6)活性蛋白
Active S100 Calcium Binding Protein A6 (S100A6)
S100-A6; 2A9; 5B10; CABP; CACY; PRA; MLN 4; Calcyclin; Growth factor-inducible protein 2A9; Prolactin receptor-associated protein
- 編號APB769Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 90%
- 等電點5.7
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure . Gene Sequencing (extract)
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SDS-PAGE圖
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Figure. Western Blot; Sample: Recombinant S100A6, Human.
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實驗
S100A6 is a calcium-binding protein, which functions as calcium sensor and modulator, contributing to cellular calcium signaling. It is reported that S100B and S100A6 differentially modulate cell survival by Interacting with distinct RAGE (Receptor for Advanced Glycation End Products). Thus a binding ELISA assay was conducted to detect the interaction of S100A6 and RAGE. Briefly, recombinant human S100A6 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RAGE-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-S100A6 mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of S100A6 and RAGE was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APB769Hu01 | S100鈣結(jié)合蛋白A6(S100A6)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB769Hu01 | S100鈣結(jié)合蛋白A6(S100A6)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB769Hu01 | S100鈣結(jié)合蛋白A6(S100A6)多克隆抗體 | WB,IHC,ICC/IF |
| LAB769Hu81 | S100鈣結(jié)合蛋白A6(S100A6)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAB769Hu71 | S100鈣結(jié)合蛋白A6(S100A6)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAB769Hu23 | S100鈣結(jié)合蛋白A6(S100A6)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB769Hu22 | S100鈣結(jié)合蛋白A6(S100A6)單克隆抗體 | WB; ICC/IF |
| MAB769Hu21 | S100鈣結(jié)合蛋白A6(S100A6)單克隆抗體 | IHC; ICC/IF |
| MAB769Hu24 | S100鈣結(jié)合蛋白A6(S100A6)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB769Hu | S100鈣結(jié)合蛋白A6(S100A6)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB769Hu | S100鈣結(jié)合蛋白A6(S100A6)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSB769Hu01 | S100鈣結(jié)合蛋白A6(S100A6)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSB769Hu01 | S100鈣結(jié)合蛋白A6(S100A6)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Journal of Proteomics | Tear proteome and protein network analyses reveal a novel pentamarker panel for tear film characterization in dry eye and meibomian gland dysfunction [PubMed: 23201116] |
| Tumour Biol | Diagnostic significance of S100A2 and S100A6 levels in sera of patients with non-small cell lung cancer [PubMed: 26361956] |
| Clinical and Translational Medicine | Serum levels of S100A6 are unaltered in patients with resectable cholangiocarcinoma [pubmed:27709523] |
| Gastroenterology | Tu1938-Micronutrients Assessment in Patients with Cirrhosis [10.1016:S0016-5085(18)33544-3] |
| Gastroenterology | Tu1942-PI3K/AKT-Mediated Upregulation of C7 Inhibits Colorectal Cancer Proliferation and Metastasis [10.1016:S0016-5085(18)33544-5] |
| Tumor?Biology | Proteomics detection of S100A6 in tumor tissue interstitial fluid and evaluation of its potential as a biomarker of cholangiocarcinoma [Pubmed:29629840] |
| scandinavian journal of clinical & laboratory investigation | Serum S100A6, S100A8, S100A9 and S100A11 proteins in colorectal neoplasia: results of a single centre prospective study [Pubmed: 31856598] |
| CELL BIOCHEMISTRY AND FUNCTION | S100A6 represses Calu?\6 lung cancer cells growth via inhibiting cell proliferation, migration, invasion and enhancing apoptosis [34008212] |
| MOLECULAR VISION | Quantification of a panel for dry-eye protein biomarkers in tears: A comparative pilot study using standard ELISA and customized microarrays [34012227] |
