欧美综合自拍亚洲综合网_欧美黑人巨大videos异族_可乐操中文字幕_亚洲成av人福利专区网站_国产强奸视频在播放_免费无码国产在线53_最近最新的免費中文字幕_抖音免费视频素材_亚洲精品熟女一二区_国产无套粉嫩白浆在线麻豆

S100鈣結(jié)合蛋白A8(S100A8)活性蛋白

Active S100 Calcium Binding Protein A8 (S100A8)

MRP8; MIF; 60B8AG; CAGA; CFAG; CGLA; CP-10; L1Ag; NIF; P8; Calgranulin A; Cystic fibrosis antigen; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8

  • S100鈣結(jié)合蛋白A8(S100A8)活性蛋白 產(chǎn)品包裝(模擬)
  • S100鈣結(jié)合蛋白A8(S100A8)活性蛋白 產(chǎn)品包裝(模擬)
  • APB792Mu61.png Figure. SDS-PAGE
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

S100?calcium-binding protein A8?(S100A8)?also known as calgranulin A, is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Besides, S100 Calcium Binding Protein A9 (S100A9) has been identified as an interactor of S100A8, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse S100A8 and recombinant rat S100A9. Briefly, biotin-linked S100A8 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to S100A9-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant mouse S100A8 and recombinant rat S100A9 was shown in Figure 1, the EC50?for this effect is 0.2 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

相關(guān)產(chǎn)品

編號(hào) 適用物種：Mus musculus (Mouse，小鼠) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPB792Mu02 S100鈣結(jié)合蛋白A8(S100A8)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPB792Mu61 S100鈣結(jié)合蛋白A8(S100A8)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB792Mu61 S100鈣結(jié)合蛋白A8(S100A8)活性蛋白 Cell?culture;?Activity?Assays.
RPB792Mu01 S100鈣結(jié)合蛋白A8(S100A8)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB792Mu01 S100鈣結(jié)合蛋白A8(S100A8)多克隆抗體 WB; IHC; ICC; IP.
PAB792Mu02 S100鈣結(jié)合蛋白A8(S100A8)多克隆抗體 WB; IHC; ICC; IP.
LAB792Mu81 S100鈣結(jié)合蛋白A8(S100A8)多克隆抗體(異硫氰酸熒光素標(biāo)記) WB; IHC; ICC; IF.
LAB792Mu71 S100鈣結(jié)合蛋白A8(S100A8)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
MAB792Mu21 S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體 WB; IHC; ICC; IP.
SEB792Mu S100鈣結(jié)合蛋白A8(S100A8)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB792Mu S100鈣結(jié)合蛋白A8(S100A8)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSB792Mu01 S100鈣結(jié)合蛋白A8(S100A8)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Biochemical and Biophysical Research Communications High circulating levels of S100A8/A9 complex (calprotectin) in male Japanese with abdominal adiposity and dysregulated expression of S100A8 and S100A9 in adipose tissues of obese mice [ScienceDirect: S0006291X1200352X]
American Journal of Reproductive Immunology Association of Increased S100A8 Serum Protein with Early Pregnancy Loss [Pubmed:25252120]
the faseb journal Soluble receptor for advanced glycation end products inhibits disease progression in autosomal dominant polycystic kidney disease by down-regulating cell proliferation [PubMed: 25934702]
J Immunol S100A8 Production in CXCR2-Expressing CD11b+ Gr-1high Cells Aggravates Hepatitis in Mice Fed a High-Fat and High-Cholesterol Diet [PubMed: 26608915]
PLoS?One Analytical Performance of ELISA Assays in Urine: One More Bottleneck towards Biomarker Validation and Clinical Implementation [Pubmed:26889680]
Journal?of?Alzheimer's?Disease Proteomics?Analysis?of?Blood?Serums?from?Alzheimer's?Disease?Patients?Using?iTRAQ?LabelingTechnology. [pubmed:27911324]
?Journal of Functional Foods Standardized ethanolic extract of the rhizome of Curcuma xanthorrhiza prevents murine ulcerative colitis by regulation of inflammation [S1756464617300208]
International?Journal?of?Molecular?Medicine Proinflammatory effects of S100A8/A9 via TLR4 and RAGE signaling pathways in BV-2 microglial cells [pubmed:28498464]
International journal of molecular sciences Immune Modulating Topical S100A8/A9 Inhibits Growth of Pseudomonas aeruginosa and Mitigates Biofilm Infection in Chronic Wounds. [pubmed:28672877]
Gastroenterology Tu1938-Micronutrients Assessment in Patients with Cirrhosis [10.1016:S0016-5085(18)33544-3]
Gastroenterology Tu1942-PI3K/AKT-Mediated Upregulation of C7 Inhibits Colorectal Cancer Proliferation and Metastasis [10.1016:S0016-5085(18)33544-5]
Journal of Biological Chemistry Signaling between pancreatic β-cells and macrophages via S100 calcium-binding protein A8 exacerbates β-cell apoptosis and islet inflammation [Pubmed:29496993]
European?review for?medical?and pharmacological?sciences S100A8/A9 promotes MMP-9 expression in the fibroblasts from cardiac rupture after myocardial infarction by inducing macrophages secreting TNFα. [Pubmed:29949169]
Foodborne Pathogens and Disease Synergistic effect of immunomodulatory S100A8/A9 and ciprofloxacin against Pseudomonas aeruginosa biofilm in a murine chronic wound model [Pubmed: 31116394]
Kidney International Reports Systemic Inflammation Precedes Microalbuminuria in Diabetes []
Cancer Management and Research iTRAQ-based quantitative protein expression profiling of biomarkers in childhood B-cell and T-cell acute lymphoblastic leukemia [Pubmed: 31440093]
scandinavian journal of clinical & laboratory investigation Serum S100A6, S100A8, S100A9 and S100A11 proteins in colorectal neoplasia: results of a single centre prospective study [Pubmed: 31856598]
ANNALS OF AGRICULTURAL AND ENVIRONMENTAL MEDICINE Analysis of novel serum markers of fibrosis and angiogenesis in patients with alcoholic liver cirrhosis []
MOLECULAR VISION Quantification of a panel for dry-eye protein biomarkers in tears: A comparative pilot study using standard ELISA and customized microarrays [34012227]
university of copenhagen Adjunctive S100A8/A9 Immunomodulation Hinders Cipro fl oxacin Resistance in []
留言咨詢