性激素結(jié)合球蛋白(SHBG)活性蛋白
Active Sex Hormone Binding Globulin (SHBG)
ABP; TEBG; SBP; Sex steroid-binding protein; Testis-specific androgen-binding protein; Testosterone-estradiol-binding globulin; Testosterone-estrogen-binding globulin
- 編號APA396Hu61
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點6.2
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure . Gene Sequencing (extract)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實驗
Sex hormone-binding globulin (SHBG) or sex steroid-binding globulin (SSBG) is a glycoprotein, a molecule that consists of a carbohydrate plus a protein, and is produced mainly in your liver. It binds to three sex hormones found in both men and women: estrogen, dihydrotestosterone (DHT) and testosterone. Its job is to transport these hormones throughout your blood to other tissues in your body. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human SHBG and bsa-conjugated testosterone(Testo) . Briefly, biotin-linked recombinant human SHBG were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to bsa-conjugated Testo -coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with HRP conjugage for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μl stop solution to the wells and read at 450nm immediately. The binding activity of SHBG and Testo was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| EPA396Hu61 | 性激素結(jié)合球蛋白(SHBG)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA396Hu62 | 性激素結(jié)合球蛋白(SHBG)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA396Hu02 | 性激素結(jié)合球蛋白(SHBG)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA396Hu01 | 性激素結(jié)合球蛋白(SHBG)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA396Hu63 | 性激素結(jié)合球蛋白(SHBG)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA396Hu61 | 性激素結(jié)合球蛋白(SHBG)活性蛋白 | Cell?culture;?Activity?Assays. |
| APA396Hu62 | 性激素結(jié)合球蛋白(SHBG)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA396Hu01 | 性激素結(jié)合球蛋白(SHBG)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA396Hu02 | 性激素結(jié)合球蛋白(SHBG)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA396Hu71 | 性激素結(jié)合球蛋白(SHBG)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAA396Hu81 | 性激素結(jié)合球蛋白(SHBG)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| MAA396Hu27 | 性激素結(jié)合球蛋白(SHBG)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA396Hu29 | 性激素結(jié)合球蛋白(SHBG)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA396Hu25 | 性激素結(jié)合球蛋白(SHBG)單克隆抗體 | WB |
| MAA396Hu24 | 性激素結(jié)合球蛋白(SHBG)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA396Hu26 | 性激素結(jié)合球蛋白(SHBG)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA396Hu22 | 性激素結(jié)合球蛋白(SHBG)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA396Hu21 | 性激素結(jié)合球蛋白(SHBG)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA396Hu23 | 性激素結(jié)合球蛋白(SHBG)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA396Hu | 性激素結(jié)合球蛋白(SHBG)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA396Hu | 性激素結(jié)合球蛋白(SHBG)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA396Hu01 | 性激素結(jié)合球蛋白(SHBG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Nutrition and Cancer | A Pilot Study Comparing the Effect of Flaxseed, Aromatase Inhibitor, and the Combination on Breast Tumor Biomarkers [Tandfonline: Source] |
| international journal of environmental research and public health | Elevated Serum Bisphenol A Level in Patients with Dilated Cardiomyopathy [PubMed: 25996886] |
| Journal of Life Science | Effects of Fermented Dandelion (Taraxacum coreanum) Extract on Male Climacteric Syndrome [SMGHBM_2016_v26n9_1063] |
| Scientific Reports | Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus [pubmed:27461373] |
| 33 | Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. [pubmed:28336389] |
| Toxicology and?Applied?Pharmacology | Progressive effects of silver nanoparticles on hormonal regulation of reproduction in male rats [pubmed:27746313] |
| 中國醫(yī)藥導(dǎo)報 | 益腎祛濁方對PCOS-IR大鼠Ghrelin、SHBG及VF的影響 [yycyzx201733003] |
| Journal of Endocrinology | Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome [Pubmed: 29535146] |
| Biomed Pharmacother | Comparative study on beneficial effects of vitamins B and D in attenuating doxorubicin induced cardiotoxicity in rats: Emphasis on calcium homeostasis [34029952] |
| Food Science and Biotechnology | Improvement of testosterone deficiency by fermented Momordica charantia extracts in aging male rats [33868755] |
| Nutrients | Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Mice [Pubmed:35057459] |
