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干細(xì)胞因子(SCF)活性蛋白

Active Stem Cell Factor (SCF)

KITLG; MGF; SF; KL1; Kitl; FPH2; Kit-Ligand; Steel Factor; Mast Cell Growth Factor; Steel factor; Familial Progressive Hyperpigmentation 2

  • 干細(xì)胞因子(SCF)活性蛋白 產(chǎn)品包裝(模擬)
  • 干細(xì)胞因子(SCF)活性蛋白 產(chǎn)品包裝(模擬)
  • APA120Hu62.jpg Figure. SDS-PAGE
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

Stem cell factor (SCF), also known as mast cell growth factor (MGF), and steel factor (SLF), plays an important role in hematopoiesis, spermatogenesis and melanogenesis. SCF has been shown to??stimulate?the proliferation of TF-1 cells. To test this effect,?TF-1 cells were seeded into triplicate wells of 96-well plates and incubated for 72h in the presence or absence of various concentrations of SCF at 37℃The growth of cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450 nm using a microplate reader after incubating the plate for 1-4 hours at 37℃. Cell proliferation of TF-1 cells after incubation with SCF for 72h observed by inverted microscope was shown in Figure 1. The dose-effect curve of SCF was shown in Figure 2. It was obvious that it significantly promoted cell proliferation of TF-1 cells. The EC50 for this effect is typically 8.1 ng/ml.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

相關(guān)產(chǎn)品

編號(hào) 適用物種：Homo sapiens (Human，人) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA120Hu02 干細(xì)胞因子(SCF)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA120Hu61 干細(xì)胞因子(SCF)活性蛋白 Cell?culture;?Activity?Assays.
EPA120Hu61 干細(xì)胞因子(SCF)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA120Hu62 干細(xì)胞因子(SCF)活性蛋白 Cell?culture;?Activity?Assays.
RPA120Hu01 干細(xì)胞因子(SCF)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA120Hu01 干細(xì)胞因子(SCF)活性蛋白 Cell?culture;?Activity?Assays.
PAA120Hu01 干細(xì)胞因子(SCF)多克隆抗體 WB; IHC; ICC; IP.
MAA120Hu22 干細(xì)胞因子(SCF)單克隆抗體 WB; IHC; ICC; IP.
MEA120Hu 干細(xì)胞因子(SCF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA120Hu 干細(xì)胞因子(SCF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA120Hu 干細(xì)胞因子(SCF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA120Hu 干細(xì)胞因子(SCF)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA120Hu01 干細(xì)胞因子(SCF)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Archives of Gynecology and Obstetrics Effect of ovarian stimulation with recombinant follicle-stimulating hormone, gonadotropin-releasing hormone agonist and antagonists, on follicular fluid stem cell factor and serum urocortin 1 levels on the day of oocyte retrieval [Pubmed: 23801011]
Medicine (Baltimore) An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer [PubMed: 26266370]
Tumor Biology Hypoxia-induced secretion of IL-13 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma [PubMed: 26695151]
Molecular?Neurobiology Middle-Aged?Diabetic?Females?and?Males?Present?Distinct?Susceptibility?to?Alzheimer?Disease-like?Pathology. [pubmed:27730513]
Oncotarget Nucleoside reverse transcriptase inhibitor-induced rat oocyte dysfunction and low fertility mediated by autophagy [Pubmed:29423092]
Journal of Environmental Research and Public Health Effects of 220 MHz Pulsed Modulated Radiofrequency Field on the Sperm Quality in Rats [Pubmed: 30974849]
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