干細(xì)胞因子受體(SCFR)活性蛋白
Active Stem Cell Factor Receptor (SCFR)
CD117; KIT; C-Kit; PBT; p145 c-Kit; C-Kit Receptor; Tyrosine-Protein Kinase; V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog; Piebald Trait
- 編號(hào)APA121Hu61
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 緩沖液成份PBS, pH7.4, containing 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)6.4
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 1354 ¥ 3384 ¥ 6768 ¥ 20304 ¥ 50760
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活性實(shí)驗(yàn)
Stem Cell Factor Receptor (SCFR), also known as c-Kit and CD117, is a widely expressed 145 kDa receptor tyrosine kinase. Binding of SCF R to SCF promotes the survival, differentiation, and mobilization of progenitor cells in multiple lineages. Mature human SCFR consists of a 499 amino acid (aa) extracellular domain (ECD) with five tandem immunoglobulin-like domains, a 21 aa transmembrane segment, and a 431 aa cytoplasmic domain with the split tyrosine kinase domain. SCFR is up-regulated on dendritic cells by Th2- or Th17-biasing stimuli, and it is required for subsequent dendritic cell induction of Th2 and Th17 responses. Besides, Epidermal Growth Factor (EGF) has been identified as an interactor of SCFR, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human SCFR and recombinant rat EGF. Briefly, biotin-linked SCFR were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to EGF-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human SCFR and recombinant rat EGF was shown in Figure 1, the EC50?for this effect is 0.09 ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月,-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。
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相關(guān)產(chǎn)品
編號(hào) | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
EPA121Hu61 | 干細(xì)胞因子受體(SCFR)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA121Hu02 | 干細(xì)胞因子受體(SCFR)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA121Hu61 | 干細(xì)胞因子受體(SCFR)活性蛋白 | Cell?culture;?Activity?Assays. |
RPA121Hu01 | 干細(xì)胞因子受體(SCFR)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA121Hu01 | 干細(xì)胞因子受體(SCFR)多克隆抗體 | WB; IHC; ICC; IP. |
MAA121Hu21 | 干細(xì)胞因子受體(SCFR)單克隆抗體 | WB; IHC; ICC; IP. |
MAA121Hu24 | 干細(xì)胞因子受體(SCFR)單克隆抗體 | WB; IHC; ICC; IP. |
MAA121Hu23 | 干細(xì)胞因子受體(SCFR)單克隆抗體 | WB; IHC; ICC; IP. |
MAA121Hu26 | 干細(xì)胞因子受體(SCFR)單克隆抗體 | WB; IHC; ICC; IP. |
MAA121Hu28 | 干細(xì)胞因子受體(SCFR)單克隆抗體 | WB; IHC; ICC; IP. |
MAA121Hu22 | 干細(xì)胞因子受體(SCFR)單克隆抗體 | WB; IHC; ICC; IP. |
SEA121Hu | 干細(xì)胞因子受體(SCFR)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA121Hu | 干細(xì)胞因子受體(SCFR)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA121Hu | 干細(xì)胞因子受體(SCFR)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Chemico-Biological Interactions | Protective effects of honokiol against methylglyoxal-induced osteoblast damage [PubMed: 26723916] |