突觸融合蛋白1A(STX1A)活性蛋白
Active Syntaxin 1A, Brain (STX1A)
STX-1A; HPC-1; STX1; P35-1; Neuron-specific antigen HPC-1
- 編號APD035Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 90%
- 等電點4.6
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 暫無報價 暫無報價 暫無報價 暫無報價 暫無報價
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實驗
Syntaxin 1A (STX1A), a member of the syntaxin family ,is a nervous system-specific membrane protein responsible for fusion.Synaptosomal Associated Protein 25kDa (SNAP25) binds to STX1A to form the SNARE complex , which is essential for the release of neurotransmitters.Thus a functional ELISA assay was conducted to detect the interaction of recombinant human STX1A and recombinant human SNAP25. Briefly, STX1A was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to SNAP25-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-STX1A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human STX1A and recombinant human SNAP25 was shown in Figure 1, the EC50?for this effect is 0.026ug/mL.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPD035Hu01 | 突觸融合蛋白1A(STX1A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APD035Hu01 | 突觸融合蛋白1A(STX1A)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAD035Hu01 | 突觸融合蛋白1A(STX1A)多克隆抗體 | WB; IHC; ICC; IP. |
| MAD035Hu21 | 突觸融合蛋白1A(STX1A)單克隆抗體 | WB; IHC; ICC; IP. |
| SED035Hu | 突觸融合蛋白1A(STX1A)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD035Hu | 突觸融合蛋白1A(STX1A)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
