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組織因子(TF)活性蛋白

Active Tissue Factor (TF)

CD142; FIII; F3; TFA; Thromboplastin; Coagulation Factor III

  • 組織因子(TF)活性蛋白 產(chǎn)品包裝(模擬)
  • 組織因子(TF)活性蛋白 產(chǎn)品包裝(模擬)
  • APA524Ra01.jpg SDS-PAGE圖
  • 組織因子(TF)活性蛋白 Figure. Western Blot; Sample: Recombinant Tissue Factor, Rat.
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

活性實驗

TF (Tissue factor) also known for platelet tissue factor, factor III, thromboplastin, or CD142, is a cell surface glyprotein present in subendothelial tissue and leukocytes. TF is necessary for the initiation of thrombin formation from the zymogen prothrombin. It has been proven that EGF can bind with the TF on the hemangioendotheliocytes. Thus a binding ELISA assay was conducted to detect the interaction of recombinant rat TF and recombinant rat EGF. Briefly, TF were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL TF were then transferred to EGF-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of TF and EGF was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。

相關產(chǎn)品

編號 適用物種：Rattus norvegicus (Rat，大鼠) 應用(僅供研究使用，不用于臨床診斷！)
RPA524Ra01 組織因子(TF)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA524Ra01 組織因子(TF)活性蛋白 Cell?culture;?Activity?Assays.
PAA524Ra01 組織因子(TF)多克隆抗體 WB; IHC; ICC; IP.
LAA524Ra81 組織因子(TF)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
LAA524Ra71 組織因子(TF)多克隆抗體(生物素標記) WB; IHC; ICC.
MAA524Ra21 組織因子(TF)單克隆抗體 WB; IHC; ICC; IP.
SEA524Ra 組織因子(TF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA524Ra 組織因子(TF)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
Peptides Calcitonin gene-related peptide-mediated antihypertensive and anti-platelet effects by rutaecarpine in spontaneously hypertensive rats [PubMed: 18625276]
Canadian Journal of Physiology and Pharmacology Increased plasma level of asymmetric dimethylarginine in hypertensive rats facilitates platelet aggregation: role of plasma tissue factor [Nrcresearchpress: y10-115]
International immunopharmacology Ruscogenin attenuates monocrotaline-induced pulmonary hypertension in rats [Pubmed: 23538027]
BioMed research international Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations [Pubmed: 24199192]
The Journal of Biological Chemistry Sphingolipid Pathway Regulates Innate Immune Responses at the Fetomaternal Interface during Pregnancy [Pubmed:25505239]
Hippokratia Thrombosis in Hemodialysis Patients; Their Association with Tissue Factor and Tissue Factor Pathway Inhibitor [PubMed: 20011089]
shock Noble-Collip Drum Trauma Induces Disseminated Intravascular Coagulation But Not Acute Coagulopathy of Trauma-Shock [PubMed: 25423126]
Perfusion Ex vivo simulation of cardiopulmonary bypass with human blood for hemocompatibility testing [PubMed: 26243277]
Molecular Nutrition & Food Research Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptors [PubMed: 25546502]
Food Funct The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice [Pubmed:26757699]
Blood Cells, Inflammation and Infection Nebulized Heparin Attenuates Pulmonary Coagulopathy and Inflammation through Alveolar Macrophages in a Rat Model of Acute Lung Injury [pubmed:29202212]
Thrombosis?andHaemostasis Nebulized anti-coagulants as a therapy for acute lung injury and acute respiratory distress syndrome [Pubmed: 29202212]
Journal of Thrombosis and Haemostasis Effects of nebulized antithrombin and heparin on inflammatory and coagulation alterations in an acute lung injury model in rats [Pubmed: 31755229]
Mediators Inflamm Amelioration of Coagulation Disorders and Inflammation by Hydrogen-Rich Solution Reduces Intestinal Ischemia/Reperfusion Injury in Rats through NF-κB … [Pubmed: 32587471]
International Journal of Clinical Practice Effect of Glucocorticoid Administration in Intravenous Pulses on Selected Parameters of the Coagulation System [Pubmed:35685509]
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