轉(zhuǎn)化生長(zhǎng)因子β2(TGFb2)活性蛋白
Active Transforming Growth Factor Beta 2 (TGFb2)
TGF-B2; TGF-Beta2; G-TSF; LAP; Cetermin; Polyergin; Latency-associated peptide; BSC-1 cell growth inhibitor; Glioblastoma-derived T-cell suppressor factor
- 編號(hào)APA218Hu01
- 物種Homo sapiens (Human,人) 相同的名稱(chēng),不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)8.9
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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活性實(shí)驗(yàn)
Transforming growth factor-beta 2 (TGFb2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. There are two named isoforms of this protein, created by alternative splicing of the same gene. Besides, Amyloid Precursor Protein (APP) has been identified as an interactor of TGFb2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TGFb2 and recombinant human APP. Briefly, TGFb2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to APP-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TGFb2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of TGFb2 and APP was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of TGFb2 with APP.
Transforming growth factor beta (TGF-β) is a multifunctional cytokine belonging to the transforming growth factor superfamily. The TGF-β superfamily includes endogenous growth inhibiting proteins; an increase in expression of TGF-β often correlates with the malignancy of many cancers and a defect in the cellular growth inhibition response to TGF-β. Its immunosuppressive functions then come to dominate, contributing to oncogenesis. To test the effect of TGF-β on inhibit HGF-dependent proliferation, HepG2 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM?including 1ng/mL HGF?prior to the addition of various concentrations of recombinant human TGF-β. After incubated for 96h,?cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. The inhibitory effect of TGF-β on HGF-dependent proliferation of HepG2 cells observed by inverted microscope was shown in Figure 2. Cell viability was assessed by CCK-8 assay after incubation with recombinant TGF-β for 96h. The result was shown in Figure 3. It was obvious that TGF-β significantly decreased cell viability of HepG2 cells. (A) HepG2 cells cultured in DMEM, stimulated with 1μg/mL TGF-β for 96h; (B) Unstimulated HepG2 cells cultured in DMEM for 96h.
Figure.The inhibitory effect of TGF-β on cell proliferation of HepG2 cells.
用法
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月,-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。
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編號(hào) | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPA218Hu01 | 轉(zhuǎn)化生長(zhǎng)因子β2(TGFb2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA218Hu01 | 轉(zhuǎn)化生長(zhǎng)因子β2(TGFb2)活性蛋白 | Cell?culture;?Activity?Assays. |
PAA218Hu01 | 轉(zhuǎn)化生長(zhǎng)因子β2(TGFb2)多克隆抗體 | WB; IHC; FCM |
RAA218Hu21 | 轉(zhuǎn)化生長(zhǎng)因子β2(TGFb2)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
MAA218Hu21 | 轉(zhuǎn)化生長(zhǎng)因子β2(TGFb2)單克隆抗體 | WB; IHC; ICC; IP. |
MAA218Hu22 | 轉(zhuǎn)化生長(zhǎng)因子β2(TGFb2)單克隆抗體 | WB; IHC; ICC; IP. |
FAA218Hu08 | 抗轉(zhuǎn)化生長(zhǎng)因子β2(TGFb2)單克隆抗體 | Flow cytometry. |
FAA218Hu06 | 抗轉(zhuǎn)化生長(zhǎng)因子β2(TGFb2)單克隆抗體 | Flow cytometry. |
FAA218Hu04 | 抗轉(zhuǎn)化生長(zhǎng)因子β2(TGFb2)單克隆抗體 | Flow cytometry. |
FAA218Hu02 | 抗轉(zhuǎn)化生長(zhǎng)因子β2(TGFb2)單克隆抗體 | Flow cytometry. |
SEA218Hu | 轉(zhuǎn)化生長(zhǎng)因子β2(TGFb2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CEA218Hu | 轉(zhuǎn)化生長(zhǎng)因子β2(TGFb2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA218Hu | 轉(zhuǎn)化生長(zhǎng)因子β2(TGFb2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
international Journal of food and nutritional sciences | EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI [Ijfans: Source] |
J Reprod Immunol | The immunomodulating effect of seminal plasma on T cells [PubMed: 25799173] |
International Journal of Chemical Studies | Studies on quality attributes of skimmed colostrum powder [P-ISSN: 2349–8528] |
JCI Insight | Angiocrine signals regulate quiescence and therapy resistance in bone metastasis [Pubmed: 31292293] |
Mol Med | Association of CASC18/miR-20a-3p/TGFB2 ceRNA axis with occult lymph node metastasis in tongue squamous cell carcinoma [34362313] |
Food Chem | Effect of convection and microwave heating on the retention of bioactive components in human milk [34896952] |