腫瘤壞死因子β(TNFb)活性蛋白
Active Tumor Necrosis Factor Beta (TNFb)
LT; LTA; TNF-B; TNFSF1; Tumor Necrosis Factor Ligand Superfamily Member 1; Lymphotoxin Alpha
- 編號APA134Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 97%
- 等電點9.3
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 1805 ¥ 4512 ¥ 9024 ¥ 27072 ¥ 67680
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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WB圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
TNF-β, a member of the tumor necrosis factor family, is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells. The biological effects of TNF-β are very similar to TNF-α, due to the similarity of molecular structure and the receptors. As reported, TNF-β could inhibit the proliferation and induce necrosis of A549 cells, and the concentration of IL-1β in cell supernatant will increase after stimulation. Therefore, A549 cells were incubated in DMEM with TNF-β (10ng/mL) for 8h, 24h, 48h, 72h, then cells were observed by inverted microscope and IL-1β was detected in the cell supernatant by ELISA .
Cell necrosis after incubation with TNF-β(10ng/mL) for 72h was shown in Figure 1.
The production of IL-1β after incubation with TNF-β (10ng/mL) for 8h is shown in Table 1.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APA134Hu01 | 腫瘤壞死因子β(TNFb)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA134Hu01 | 腫瘤壞死因子β(TNFb)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA134Hu01 | 腫瘤壞死因子β(TNFb)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA134Hu22 | 腫瘤壞死因子β(TNFb)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA134Hu21 | 腫瘤壞死因子β(TNFb)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA134Hu | 腫瘤壞死因子β(TNFb)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA134Hu | 腫瘤壞死因子β(TNFb)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Journal of Applied Physiology | Simvastatin attenuates sympathetic hyperinnervation to prevent atrial fibrillation during the postmyocardial infarction remodeling process [PubMed: 22984252] |
| Journal of Translational Medicine | Effectiveness of pure argon for renal transplant preservation in a preclinical pig model of heterotopic autotransplantation [Pubmed:26847569] |
| International Journal of Stem Cell Research and Transplantation | Protective Role of Bone Marrow Derived Mesenchymal Stem Cells-Conditioned Medium in the Infarcted Myocardium: The Potential Role of Selected Cytokines [IJST-2328-3548-04-803] |
| Probiotics Antimicrob Proteins | Effect of Multi-Microbial Probiotic Formulation Bokashi on Pro-and Anti-Inflammatory Cytokines Profile in the Serum, Colostrum and Milk of Sows, and in a?… [Pubmed:29305686] |
| Parasites & Vectors | Fasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism?… [Pubmed: 32680553] |
| Microbiome changes in patients with chronic heart failure with preserved ejection fraction correlate with fibrosis markers: Description of a Russian cohort [] | |
| BMC MUSCULOSKELETAL DISORDERS | Elevated lymphotoxin-|á (TNF|?) is associated with intervertebral disc degeneration [33441130] |
