腫瘤壞死因子受體超家族成員1B(TNFRSF1B)活性蛋白
Active Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B)
CD120B; P75; TBPII; TNF-R-II; TNF-R75; TNFBR; TNFR2; TNFR80; P75tnfr; Tumor necrosis factor receptor 2; p80 TNF-alpha receptor; Etanercept
- 編號APB504Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 95%
- 等電點4.9
- 應用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. Gene Sequencing (Extract)
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Figure. SDS-PAGE
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Figure. Western Blot
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) , also known as tumor necrosis factor receptor 2 (TNFR2) and CD120b, is a membrane receptor that binds tumor necrosis factor-alpha (TNFα). This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Besides, Tumor Necrosis Factor Alpha (TNFa) has been identified as an interactor of TNFRSF1B, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TNFRSF1B and recombinant human TNFa. Briefly, TNFRSF1B were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TNFa-coated microtiter wells and incubated for 1h at 37 ℃. Wells were washed with PBST and incubated for 1h with anti-TNFRSF1B pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 ℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF1B and TNFa was shown in Figure 1, the EC50 was 0.0470~0.085 ug/ml.
用法
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| EPB504Hu61 | 腫瘤壞死因子受體超家族成員1B(TNFRSF1B)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB504Hu01 | 腫瘤壞死因子受體超家族成員1B(TNFRSF1B)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB504Hu01 | 腫瘤壞死因子受體超家族成員1B(TNFRSF1B)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB504Hu01 | 腫瘤壞死因子受體超家族成員1B(TNFRSF1B)多克隆抗體 | WB; IHC; ICC; IP. |
| FAB504Hu51 | 抗腫瘤壞死因子受體超家族成員1B(TNFRSF1B)多克隆抗體(別藻藍蛋白標記) | FCM |
| MAB504Hu21 | 腫瘤壞死因子受體超家族成員1B(TNFRSF1B)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB504Hu | 腫瘤壞死因子受體超家族成員1B(TNFRSF1B)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB504Hu | 腫瘤壞死因子受體超家族成員1B(TNFRSF1B)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
