含纈酪肽蛋白(VCP)活性蛋白
Active Valosin Containing Protein (VCP)
IBMPFD; p97; TERA; Transitional Endoplasmic Reticulum ATPase; 15S Mg(2+)-ATPase p97 subunit
- 編號APC601Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 95%
- 等電點(diǎn)5.9
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 1608 ¥ 4020 ¥ 8040 ¥ 24120 ¥ 60300
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活性實(shí)驗(yàn)
VCP is an ATPase Associated with diverse cellular Activities (AAA ) that has a key role in maintaining cellular homeostasis. VCP is ubiquitously expressed in tissues. At cellular level, VCP functions in many cellular compartments; VCP localizes mainly in the cytoplasm, while a smaller fraction binds to organelles or localizes in the nucleus, where it is implicated in different pathways. VCP assembles and acts as a homo-hexamer, in which each monomer is structured in an N-terminal domain that interacts with adaptors and co-factors; in two ATPase domains, D1 and D2, that by hydrolyzing ATP concur, respectively, in the hexamer formation and in supporting VCP activity; and in a C-terminal domain that binds to a small subset of co-factors and adaptors cooperating with D2 activity. The main role of VCP is to recognize and to extract ubiquitinated proteins from membranes, protein complexes, protein aggregates, or chromatin, modulating their ubiquitination or de-ubiquitination, and to enhance their degradation through the UPS or the autophagic pathway. The activity of recombinant human VCP was measured by its ability to hydrolyze the substrate ATP to phosphate which was detected by the malachite green phosphate detection Kit (Beyotime # S0196M). The reaction was performed in the assay buffer 20 mM HEPES, 13 mM NaCl and 1% glycerol, pH 8.5, initiated by addition 27 μL of various concentrations of VCP (diluted by assay buffer) to 3 μL of 100 mM ATP and 100 mM MgCl2 mixture. The final well serves as a negative control with no VCP, replaced with 27 μL assay buffer. Incubated at 37℃ for 5min, then stop the reaction by quickly freezing samples in the dry ice and ethanol. 4 ul samples was added to 196 ul assay buffer after thawing at room temperature followed by adding 70 ul phosphate detection agent and incubated at room temperature for 30min, then read at a wavelength of 630 nm. The specific activity of recombinant human VCP is >1000 pmol/min/μg.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個(gè)月,-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPC601Hu01 | 含纈酪肽蛋白(VCP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APC601Hu01 | 含纈酪肽蛋白(VCP)活性蛋白 | Cell?culture;?Activity?Assays. |
PAC601Hu01 | 含纈酪肽蛋白(VCP)多克隆抗體 | WB,IHC |
MAC601Hu21 | 含纈酪肽蛋白(VCP)單克隆抗體 | WB; IHC; ICC; IP. |
MEC601Hu | 含纈酪肽蛋白(VCP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SEC601Hu | 含纈酪肽蛋白(VCP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMC601Hu | 含纈酪肽蛋白(VCP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |