酸性神經(jīng)鞘磷脂酶(ASM)活性蛋白
Active Acid Sphingomyelinase (ASM)
SMPD1; NPD; SMase; aSMase; Sphingomyelin Phosphodiesterase 1,Acid Lysosomal; Simply Sphingomyelinase
- 編號APB360Hu61
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份磷酸鹽緩沖液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性狀凍干粉
- 純度> 95%
- 等電點(diǎn)7.2
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure . Gene Sequencing (extract)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Sphingomyelin phosphodiesterase 1 (SMPD1), also known as acid sphingomyelinase (ASM), belongs to the sphingomyelin phosphodiesterase family. The protein Converts sphingomyelin to ceramide. ASM also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol.
Thus, the recombinant human ASM activity was measured by its ability to hydrolyze 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HDA-PC) to p-Nitrophenol. The reaction was performed in 50 mM MES, 0.5 μM ZnCl2, pH 7.5 ( Assay Buffer), initiated by addition 50 μL of various concentrations of ASM (diluted with Assay Buffer) to 50 μL of 1 mM Substrate HDA-PC ( 50 mM stock solution in methanol, diluted with Assay Buffer). Incubated at room temperature for 20 minutes in the dark, then read at a wavelength of 405 nm.
One unit of enzyme activity is defined as the 1μg of enzyme required to convert 1pmol of HDA-PC to p-Nitrophenol in 1min at 37°C. The specific activity of recombinant human ASM is >1000 pmol/min/μg.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| EPB360Hu61 | 酸性神經(jīng)鞘磷脂酶(ASM)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB360Hu01 | 酸性神經(jīng)鞘磷脂酶(ASM)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB360Hu61 | 酸性神經(jīng)鞘磷脂酶(ASM)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAB360Hu01 | 酸性神經(jīng)鞘磷脂酶(ASM)多克隆抗體 | WB; IHC; ICC; IP. |
| MAB360Hu22 | 酸性神經(jīng)鞘磷脂酶(ASM)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB360Hu | 酸性神經(jīng)鞘磷脂酶(ASM)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEB360Hu | 酸性神經(jīng)鞘磷脂酶(ASM)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB360Hu | 酸性神經(jīng)鞘磷脂酶(ASM)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Blood.? | Acid sphingomyelinase is activated in sickle cell erythrocytes and contributes to inflammatory microparticle generation in SCD [Pubmed:25075126] |
| Journal of Clinical Lipidology | Increase in acid sphingomyelinase level in human retinal endothelial cells and CD34+ circulating angiogenic cells isolated from diabetic individuals is associated with dysfunctional retinal vasculature and vascular repair process in diabetes [10.1016/j.jacl.2017.03.007] |
| Diabetes & metabolism | Fenofibrate decreases plasma ceramide in type 2 diabetes patients: A novel marker of CVD? [pubmed:28499696] |
| INFLAMMATION | Acid Sphingomyelinase and Acid |?-Glucosidase 1 Exert Opposite Effects on Interleukin-1|?-Induced Interleukin 6 Production in Rheumatoid Arthritis Fibroblast-Like Synoviocytes [33665756] |
| Indian J Ophthalmol | Evaluation of sphingolipid metabolism on diabetic retinopathy [34708809] |
