甘露糖結合蛋白(MBL)活性蛋白
Active Mannose Binding Lectin (MBL)
MBL2; COLEC1; HSMBPC; MBP1; MBP; Collectin-1; Mannose-binding protein C; Mannan Binding Protein; Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)
- 編號APB480Hu02
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點5.8
- 應用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產品包裝(模擬)
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產品包裝(模擬)
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Gene sequencing
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SDS-PAGE圖
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Figure. Western Blot;
Sample: Recombinant MBL, Human.
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通過ISO 9001、ISO 13485質量體系認證
活性實驗
MBL2 (Mannose-binding protein C) is a calcium-dependent lectin involved in innate immune defense, which binds mannose, fucose and N-acetylglucosamine on different microorganisms, therefore results in activation of the lectin pathway of the complement system. It has been proven that MASP-2 (Mannan-binding lectin serine protease 2) forms complexes with the pattern recognition molecules MBL2, triggers the activation of the complement system. Thus, a functional binding ELISA assay was constructed to detect the association of rhMBL2 with MASP2. Briefly, rhMBL2 were diluted serially in 10mM Tris-HCl, 1M NaCl, 5mM CaCl2, and 0.05%Triton X-100 (pH 7.4). Duplicate samples of 100uL were then transferred to MASP2-coated microtiter wells and incubated for 2h at 37oC. Wells were washed with PBST and incubated for 1h with anti-MBL2 mAb, then aspirated and washed 3 times. After incubation with HRP labeled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated for 15-25 minutes at 37oC. Finally, add 50μL stop solution to the wells and read at 450nm immediately.
The binding activity of MBL2 with MASP2 was shown in Figure 1 and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPB480Hu02 | 甘露糖結合蛋白(MBL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB480Hu02 | 甘露糖結合蛋白(MBL)活性蛋白 | Cell?culture;?Activity?Assays. |
| APB480Hu61 | 甘露糖結合蛋白(MBL)活性蛋白 | Cell?culture;?Activity?Assays. |
| EPB480Hu61 | 甘露糖結合蛋白(MBL)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB480Hu03 | 甘露糖結合蛋白(MBL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB480Hu01 | 甘露糖結合蛋白(MBL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB480Hu02 | 甘露糖結合蛋白(MBL)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB480Hu01 | 甘露糖結合蛋白(MBL)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB480Hu03 | 甘露糖結合蛋白(MBL)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB480Hu71 | 甘露糖結合蛋白(MBL)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAB480Hu22 | 甘露糖結合蛋白(MBL)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB480Hu | 甘露糖結合蛋白(MBL)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEB480Hu | 抗甘露糖結合蛋白抗體(Anti-MBL)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| LMB480Hu | 甘露糖結合蛋白(MBL)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB480Hu01 | 甘露糖結合蛋白(MBL)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| American Journal of Nephrology | Analysis of the Urine Proteome of Human Contrast-Induced Kidney Injury Using Two-Dimensional Fluorescence Differential Gel Electrophoresis/Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry/Liquid Chromatography Mass Spectrometry. [Karger: 000255439] |
| Current Eye Research | Serum Levels and H/L Gene Polymorphism of Mannose-Binding Lectin in Primary Open Angle Glaucoma [Ingenta: art00007] |
| Journal of Clinical Immunology | Complement activation contributes to the injury and outcome of kidney in human anti-glomerular basement membrane disease. [PubMed: 22941511] |
| Clinical and Experimental Immunology | Urinary mannose-binding lectin is a biomarker for predicting the progression of immunoglobulin (Ig)A nephropathy [PubMed: PMC3406374] |
| Clin J Am Soc Nephrol. | Alternative Complement Pathway Activation Products in Urine and Kidneys of Patients with ANCA-Associated GN [Pubmed: 24115193] |
| ACTA TROPICA | Novel findings on the role of ficolins and colectins in the innate response against Leishmania braziliensis [Pubmed: 32827456] |
| PLoS One | Complement activation profile of patients with primary focal segmental glomerulosclerosis [Pubmed: 32569286] |
| LIFE SCIENCES | The lectin pathway of complement and the initial recognition of Leishmania infantum promastigotes [34242658] |
